Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells. (January 2019)

• Record Type: Journal Article
• Authors: Saikia, Mridusmita; Burnham, Philip; Keshavjee, Sara; Wang, Michael; Heyang, Michael; Moral-Lopez, Pablo; Hinchman, Meleana; Danko, Charles; Parker, John; De Vlaminck, Iwijn
• Is Part Of: Nature methods. Volume 16:Number 1(2019)
• Page Start: 59
• Page End: 62
• Publication Date: 2019-01
• Subjects: Life sciences -- Methodology -- Periodicals; Life sciences -- Research -- Periodicals; Biology -- Methodology -- Periodicals; Biology -- Research -- Periodicals; 570.72
• DOI: 10.1038/s41592-018-0259-9
• Languages: English
• ISSNs: 1548-7091

Abstract

Abstract We describe droplet-assisted RNA targeting by single-cell sequencing (DART-seq), a versatile technology that enables multiplexed amplicon sequencing and transcriptome profiling in single cells. We applied DART-seq to simultaneously characterize the non-A-tailed transcripts of a segmented dsRNA virus and the transcriptome of the infected cell. In addition, we used DART-seq to simultaneously determine the natively paired, variable region heavy and light chain amplicons and the transcriptome of B lymphocytes. DART-seq alters droplet sequencing in a simple and flexible way to simultaneously profile the transcriptome and multiplexed targeted RNAs, such as viral transcripts and immunoglobulin chains, in single cells.