Anoctamin-1 affects the migration and invasion of anaplastic thyroid carcinoma cells. Issue 4 (4th July 2019)

• Record Type: Journal Article
• Authors: Kim, Jae-Young; Youn, Hwa Young; Choi, June; Baek, Seung Kuk; Kwon, Soon Young; Eun, Bok Kee; Park, Jae-Yong; Oh, Kyoung Ho
• Is Part Of: Animal cells and systems. Volume 23:Issue 4(2019)
• Page Start: 294
• Page End: 301
• Publication Date: 2019-07-04
• Subjects: Anoctamin -- thyroid cancer -- invasion -- migration; Systems biology -- Periodicals; Biology -- Periodicals; Cytology -- Periodicals; Zoology -- Periodicals; 590
• DOI: 10.1080/19768354.2019.1614981
• Languages: English
• ISSNs: 1976-8354

Abstract

ABSTRACT: Anaplastic thyroid carcinoma (ATC) is a rare malignancy with very poor prognosis. The exact cause underlying its strong aggressive nature is not clear. Here, we discovered the elevated expression of anoctamin-1 (ANO1; Ca 2+ -activated Cl − channels) in advanced-stage ATC tissue. Using different ATC cell lines, the degree of expression of ANO1 was found to be related to the degree of ATC cell invasion by quantitative reverse transcription polymerase chain reaction and western blotting. Suppression of ANO1 activity either by selective inhibitor (T16Ainh-A01) or by siRNA significantly attenuated the migration and invasion of ATC cells. In conclusion, ANO1 appears to increase the ability of ATC cells to invade and migrate. Our results also suggest that the expression of ANO1 in patients with ATC may be helpful in predicting the prognosis of ATC.