Flow Cytometric Immunophenotypic Assessment of T-Cell Clonality by Vβ Repertoire Analysis: Detection of T-Cell Clonality at Diagnosis and Monitoring of Minimal Residual Disease Following Therapy. Issue 6 (August 2015)

• Record Type: Journal Article
• Authors: Tembhare, Prashant; Yuan, Constance M.; Xi, Liqiang; Morris, John C.; Liewehr, David; Venzon, David; Janik, John E.; Raffeld, Mark; Stetler-Stevenson, Maryalice
• Is Part Of: American journal of clinical pathology. Volume 135:Issue 6(2011)
• Page Start: 890
• Page End: 900
• Publication Date: 2015-08
• Subjects: T-cell clonality -- Flow cytometry -- Vβ repertoire; Diagnosis, Laboratory -- Periodicals; Pathology -- Periodicals; 616.07
• DOI: 10.1309/AJCPV2D1DDSGJDBW
• Languages: English
• ISSNs: 0002-9173

Abstract

Abstract : Flow cytometric T-cell receptor (TCR)-Vβ repertoire analysis (TCR-Vβ -R) is a sensitive method for detection of T-cell clonality; however, no uniform approach exists to define clonality in neoplastic T cells. TCR-Vβ -R was evaluated in patients with a diagnosis of T-cell neoplasia in initial diagnostic specimens from 41 patients and for minimal residual disease (MRD) monitoring in 61 sequential samples from 14 patients with mature T-cell neoplasia. Gating strategies and criteria for detection of T-cell clonality were determined. In all 41 initial specimens, T-cell clonality was demonstrated via TCR-Vβ -R. The frequency of Vβ usage was consistent with random neoplastic transformation of TCR-Vβ subsets. MRD was successfully detected in follow-up samples from all 14 patients evaluated, Furthermore, MRD after therapy was quantitated in 48 peripheral blood specimens. TCR-Vβ -R analysis is a sensitive method for detection of T-cell clonality and is useful for diagnosis and MRD detection in multiple specimen types.