A target-driven DNA-based molecular machine for rapid and homogeneous detection of arginine-vasopressin. Issue 3 (11th December 2019)

• Record Type: Journal Article
• Authors: Tan, Haocheng; Chen, Lu; Li, Xinyi; Li, Mengyuan; Zhao, Meiping
• Is Part Of: Analyst. Volume 145:Issue 3(2020)
• Page Start: 880
• Page End: 886
• Publication Date: 2019-12-11
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c9an02060h
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A DNA-based molecular machine triggered only by a specific target neuropeptide has been developed and integrated with fluorescence signal amplification system. It enabled rapid and sensitive detection of the target neuropeptide in biological matrices. Abstract : Rapid detection of physiological changes of neuropeptides is of great importance as they are involved in a wide range of physiological processes and behaviors. Abnormalities in their expression level are correlated with various neurological diseases. However, current methods such as radioimmunoassay, enzyme-linked immunosorbent assays and liquid chromatography tandem mass spectrometry relied on cumbersome operation steps and could not rapidly provide the information of their concentration fluctuations. Thus motivated, we developed a target-driven DNA-based molecular machine that could be triggered only in the presence of a specific target neuropeptide. Using arginine-vasopressin (AVP) as a model neuropeptide, we integrated the DNA-based molecular machine with fluorescence signal transduction and amplification technology. The assay was rapid and homogeneous, which offered a linear range of 75–700 pM and a limit-of-detection as low as 75 pM. It holds great potential for further applications in real-time monitoring of the variations of the AVP level in biological samples.