Pseudomonas aeruginosa zinc uptake in chelating environment is primarily mediated by the metallophore pseudopaline. Issue 1 (December 2017)

• Record Type: Journal Article
• Authors: Lhospice, Sébastien; Gomez, Nicolas; Ouerdane, Laurent; Brutesco, Catherine; Ghssein, Ghassan; Hajjar, Christine; Liratni, Ahmed; Wang, Shuanglong; Richaud, Pierre; Bleves, Sophie; Ball, Geneviève; Borezée-Durant, Elise; Lobinski, Ryszard; Pignol, David; Arnoux, Pascal; Voulhoux, Romé
• Is Part Of: Scientific reports. Volume 7:Issue 1(2017)
• Page Start: 1
• Page End: 10
• Publication Date: 2017-12
• Subjects: Natural history -- Research -- Periodicals; Biology -- Research -- Periodicals; Physical sciences -- Research -- Periodicals; Earth sciences -- Research -- Periodicals; Environmental sciences -- Research -- Periodicals; 502.85
• DOI: 10.1038/s41598-017-16765-9
• Languages: English
• ISSNs: 2045-2322

Abstract

Abstract Metal uptake is vital for all living organisms. In metal scarce conditions a common bacterial strategy consists in the biosynthesis of metallophores, their export in the extracellular medium and the recovery of a metal-metallophore complex through dedicated membrane transporters. Staphylopine is a recently described metallophore distantly related to plant nicotianamine that contributes to the broad-spectrum metal uptake capabilities ofStaphylococcus aureus . Here we characterize a four-gene operon (PA4837 –PA4834 ) inPseudomonas aeruginosa involved in the biosynthesis and trafficking of a staphylopine-like metallophore named pseudopaline. Pseudopaline differs from staphylopine with regard to the stereochemistry of its histidine moiety associated with an alpha ketoglutarate moiety instead of pyruvate.In vivo, the pseudopaline operon is regulated by zinc through the Zur repressor. The pseudopaline system is involved in nickel uptake in poor media, and, most importantly, in zinc uptake in metal scarce conditions mimicking a chelating environment, thus reconciling the regulation of thecnt operon by zinc with its function as the main zinc importer under these metal scarce conditions.