Sharpening emitter localization in front of a tuned mirror. Issue 1 (December 2018)

• Record Type: Journal Article
• Authors: Heil, Hannah; Schreiber, Benjamin; Götz, Ralph; Emmerling, Monika; Dabauvalle, Marie-Christine; Krohne, Georg; Höfling, Sven; Kamp, Martin; Sauer, Markus; Heinze, Katrin
• Is Part Of: Light, science & applications. Volume 7:Issue 1(2018:Jan.)
• Page Start: 1
• Page End: 8
• Publication Date: 2018-12
• Subjects: Optics -- Research -- Periodicals; Photonics -- Periodicals; 535.05
• DOI: 10.1038/s41377-018-0104-z
• Languages: English
• ISSNs: 2047-7538

Abstract

Abstract Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1 . Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tuned mirror2–4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.