Organelle DNA degradation contributes to the efficient use of phosphate in seed plants. (December 2018)

• Record Type: Journal Article
• Authors: Takami, Tsuneaki; Ohnishi, Norikazu; Kurita, Yuko; Iwamura, Shoko; Ohnishi, Miwa; Kusaba, Makoto; Mimura, Tetsuro; Sakamoto, Wataru
• Is Part Of: Nature plants. Volume 4:Number 12(2018)
• Page Start: 1044
• Page End: 1055
• Publication Date: 2018-12
• Subjects: Plants -- Periodicals; 580.5; 580 21
• DOI: 10.1038/s41477-018-0291-x
• Languages: English
• ISSNs: 2055-0278

Abstract

Abstract Mitochondria and chloroplasts (plastids) both harbour extranuclear DNA that originates from the ancestral endosymbiotic bacteria. These organelle DNAs (orgDNAs) encode limited genetic information but are highly abundant, with multiple copies in vegetative tissues, such as mature leaves. Abundant orgDNA constitutes a substantial pool of organic phosphate along with RNA in chloroplasts, which could potentially contribute to phosphate recycling when it is degraded and relocated. However, whether orgDNA is degraded nucleolytically in leaves remains unclear. In this study, we revealed the prevailing mechanism in which organelle exonuclease DPD1 degrades abundant orgDNA during leaf senescence. The DPD1 degradation system is conserved in seed plants and, more remarkably, we found that it was correlated with the efficient use of phosphate when plants were exposed to nutrient-deficient conditions. The loss of DPD1 compromised both the relocation of phosphorus to upper tissues and the response to phosphate starvation, resulting in reduced plant fitness. Our findings highlighted that DNA is also an internal phosphate-rich reservoir retained in organelles since their endosymbiotic origin. In certain types of plant cells, organelle DNA accounts for a substantial proportion of cellular total DNA. Thus, it is hypothesized that organelle DNA could not only serve as the genetic material but also function as a 'nutrient reservoir'. Now, the researchers demonstrate a mechanism involved in chloroplast DNA degradation and phosphorus recycling during leaf senescence.