An interactive framework for whole-brain maps at cellular resolution. (January 2018)

• Record Type: Journal Article
• Authors: Fürth, Daniel; Vaissière, Thomas; Tzortzi, Ourania; Xuan, Yang; Märtin, Antje; Lazaridis, Iakovos; Spigolon, Giada; Fisone, Gilberto; Tomer, Raju; Deisseroth, Karl; Carlén, Marie; Miller, Courtney; Rumbaugh, Gavin; Meletis, Konstantinos
• Is Part Of: Nature neuroscience. Volume 21:Number 1(2018)
• Page Start: 139
• Page End: 149
• Publication Date: 2018-01
• Subjects: Neurosciences -- Periodicals; 573.8
• DOI: 10.1038/s41593-017-0027-7
• Languages: English
• ISSNs: 1097-6256

Abstract

Abstract To deconstruct the architecture and function of brain circuits, it is necessary to generate maps of neuronal connectivity and activity on a whole-brain scale. New methods now enable large-scale mapping of the mouse brain at cellular and subcellular resolution. We developed a framework to automatically annotate, analyze, visualize and easily share whole-brain data at cellular resolution, based on a scale-invariant, interactive mouse brain atlas. This framework enables connectivity and mapping projects in individual laboratories and across imaging platforms, as well as multiplexed quantitative information on the molecular identity of single neurons. As a proof of concept, we generated a comparative connectivity map of five major neuron types in the corticostriatal circuit, as well as an activity-based map to identify hubs mediating the behavioral effects of cocaine. Thus, this computational framework provides the necessary tools to generate brain maps that integrate data from connectivity, neuron identity and function. The authors present a new computational approach to automatically annotate, analyze, visualize and easily share whole-brain datasets at cellular resolution, based on a scale-invariant and interactive mouse brain reference atlas. The authors applied this framework to define the organization and cocaine-induced activity of corticostriatal circuits.