MicroRNA Expression in Ethnic Specific Early Stage Breast Cancer: an Integration and Comparative Analysis. Issue 1 (December 2017)

• Record Type: Journal Article
• Authors: Nassar, Farah; Talhouk, Rabih; Zgheib, Nathalie; Tfayli, Arafat; El Sabban, Maya; El Saghir, Nagi; Boulos, Fouad; Jabbour, Mark; Chalala, Claude; Boustany, Rose-Mary; Kadara, Humam; Zhang, Zhou; Zheng, Yinan; Joyce, Brian; Hou, Lifang; Bazarbachi, Ali; Calin, George; Nasr, Rihab
• Is Part Of: Scientific reports. Volume 7:Issue 1(2017)
• Page Start: 1
• Page End: 12
• Publication Date: 2017-12
• Subjects: Natural history -- Research -- Periodicals; Biology -- Research -- Periodicals; Physical sciences -- Research -- Periodicals; Earth sciences -- Research -- Periodicals; Environmental sciences -- Research -- Periodicals; 502.85
• DOI: 10.1038/s41598-017-16978-y
• Languages: English
• ISSNs: 2045-2322

Abstract

Abstract Breast cancer (BC) has a higher incidence in young Lebanese woman as compared to the West. We assessed the microRNA (miRNA) microarray profile of tissues derived from Lebanese patients with early BC and performed mRNA-miRNA integration analysis. 173 miRNAs were significantly dysregulated in 45 BC versus 17 normal adjacent breast tissues, including 74 with a fold change more than two of which 17 were never reported before in cancer. Integration analysis of mRNA-miRNA microarray data revealed a potential role of 51 dysregulated miRNA regulating 719 tumor suppressive or oncogenic mRNA associated with increased proliferation and decreased migration and invasion. We then performed a comparative miRNA microarray profile analysis of BC tissue between these 45 Lebanese and 197 matched American BC patients. Notably, Lebanese BC patients had 21 exclusively dysregulated miRNA (e.g. miR-31, 362-3p, and 663) and 4 miRNA with different expression manner compared to American patients (e.g. miR-1288-star and 324-3p). Some of these differences could reflect variation in patient age at diagnosis or ethnic variation affecting miRNA epigenetic regulation or sequence of miRNA precursors. Our data provide a basis for genetic/epigenetic investigations to explore the role of miRNA in early stage BC in young women, including ethnic specific differences.