Closed-loop all-optical interrogation of neural circuits in vivo. (December 2018)

• Record Type: Journal Article
• Authors: Zhang, Zihui; Russell, Lloyd; Packer, Adam; Gauld, Oliver; Häusser, Michael
• Is Part Of: Nature methods. Volume 15:Number 12(2018)
• Page Start: 1037
• Page End: 1040
• Publication Date: 2018-12
• Subjects: Life sciences -- Methodology -- Periodicals; Life sciences -- Research -- Periodicals; Biology -- Methodology -- Periodicals; Biology -- Research -- Periodicals; 570.72
• DOI: 10.1038/s41592-018-0183-z
• Languages: English
• ISSNs: 1548-7091

Abstract

Abstract Understanding the causal relationship between neural activity and behavior requires the ability to perform rapid and targeted interventions in ongoing activity. Here we describe a closed-loop all-optical strategy for dynamically controlling neuronal activity patterns in awake mice. We rapidly tailored and delivered two-photon optogenetic stimulation based on online readout of activity using simultaneous two-photon imaging, thus enabling the manipulation of neural circuit activity 'on the fly' during behavior. A closed-loop all-optical strategy allows manipulation of neurons on the basis of their ongoing activity and can be used to clamp neuronal activity to a preset level, boost sensory-evoked activity or yoke together the activity of trigger and target neurons.