Cell-type-specific and projection-specific brain-wide reconstruction of single neurons. (December 2018)

• Record Type: Journal Article
• Authors: Lin, Rui; Wang, Ruiyu; Yuan, Jing; Feng, Qiru; Zhou, Youtong; Zeng, Shaoqun; Ren, Miao; Jiang, Siqi; Ni, Hong; Zhou, Can; Gong, Hui; Luo, Minmin
• Is Part Of: Nature methods. Volume 15:Number 12(2018)
• Page Start: 1033
• Page End: 1036
• Publication Date: 2018-12
• Subjects: Life sciences -- Methodology -- Periodicals; Life sciences -- Research -- Periodicals; Biology -- Methodology -- Periodicals; Biology -- Research -- Periodicals; 570.72
• DOI: 10.1038/s41592-018-0184-y
• Languages: English
• ISSNs: 1548-7091

Abstract

Abstract We developed a dual-adeno-associated-virus expression system that enables strong and sparse labeling of individual neurons with cell-type and projection specificity. We demonstrated its utility for whole-brain reconstruction of midbrain dopamine neurons and striatum-projecting cortical neurons. We further extended the labeling method for rapid reconstruction in cleared thick brain sections and simultaneous dual-color labeling. This labeling system may facilitate the process of generating mesoscale single-neuron projectomes of mammalian brains. An AAV-based platform achieves sparse yet bright labeling of neurons with cell-type specificity. This technology will facilitate the reconstruction of neurons in the mouse brain.