Brain-wide circuit interrogation at the cellular level guided by online analysis of neuronal function. (December 2018)

• Record Type: Journal Article
• Authors: Vladimirov, Nikita; Wang, Chen; Höckendorf, Burkhard; Pujala, Avinash; Tanimoto, Masashi; Mu, Yu; Yang, Chao-Tsung; Wittenbach, Jason; Freeman, Jeremy; Preibisch, Stephan; Koyama, Minoru; Keller, Philipp; Ahrens, Misha
• Is Part Of: Nature methods. Volume 15:Number 12(2018)
• Page Start: 1117
• Page End: 1125
• Publication Date: 2018-12
• Subjects: Life sciences -- Methodology -- Periodicals; Life sciences -- Research -- Periodicals; Biology -- Methodology -- Periodicals; Biology -- Research -- Periodicals; 570.72
• DOI: 10.1038/s41592-018-0221-x
• Languages: English
• ISSNs: 1548-7091

Abstract

Abstract Whole-brain imaging allows for comprehensive functional mapping of distributed neural pathways, but neuronal perturbation experiments are usually limited to targeting predefined regions or genetically identifiable cell types. To complement whole-brain measures of activity with brain-wide manipulations for testing causal interactions, we introduce a system that uses measured activity patterns to guide optical perturbations of any subset of neurons in the same fictively behaving larval zebrafish. First, a light-sheet microscope collects whole-brain data that are rapidly analyzed by a distributed computing system to generate functional brain maps. On the basis of these maps, the experimenter can then optically ablate neurons and image activity changes across the brain. We applied this method to characterize contributions of behaviorally tuned populations to the optomotor response. We extended the system to optogenetically stimulate arbitrary subsets of neurons during whole-brain imaging. These open-source methods enable delineating the contributions of neurons to brain-wide circuit dynamics and behavior in individual animals. Imaging of neuronal activity across the whole zebrafish brain in combination with online analysis allows for manipulating neuronal activity according to function. This approach is used to ablate or activate neurons in fictively swimming zebrafish larvae.