Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells. Issue 1 (December 2017)

• Record Type: Journal Article
• Authors: Requena, Sebastian; Ponomarchuk, Olga; Castillo, Marlius; Rebik, Jonathan; Brochiero, Emmanuelle; Borejdo, Julian; Gryczynski, Ignacy; Dzyuba, Sergei; Gryczynski, Zygmunt; Grygorczyk, Ryszard; Fudala, Rafal
• Is Part Of: Scientific reports. Volume 7:Issue 1(2017)
• Page Start: 1
• Page End: 6
• Publication Date: 2017-12
• Subjects: Natural history -- Research -- Periodicals; Biology -- Research -- Periodicals; Physical sciences -- Research -- Periodicals; Earth sciences -- Research -- Periodicals; Environmental sciences -- Research -- Periodicals; 502.85
• DOI: 10.1038/s41598-017-17037-2
• Languages: English
• ISSNs: 2045-2322

Abstract

Abstract Abnormalities of mucus viscosity play a critical role in the pathogenesis of several respiratory diseases, including cystic fibrosis. Currently, there are no approaches to assess the rheological properties of mucin granule matrices in live cells. This is the first example of the use of a molecular rotor, a BODIPY dye, to quantitatively visualize the viscosity of intragranular mucin matrices in a large population of individual granules in differentiated primary bronchial epithelial cells using fluorescence lifetime imaging microscopy.