Meta-omics in Inflammatory Bowel Disease Research: Applications, Challenges, and Guidelines. (22nd January 2016)

• Record Type: Journal Article
• Authors: Valles-Colomer, Mireia; Darzi, Youssef; Vieira-Silva, Sara; Falony, Gwen; Raes, Jeroen; Joossens, Marie
• Is Part Of: Journal of Crohn's and colitis. Volume 10:Number 6(2016:Jun.)
• Page Start: 735
• Page End: 746
• Publication Date: 2016-01-22
• Subjects: Metagenomics -- metatranscriptomics -- metaproteomics -- 16S -- meta-omics -- microbiota; Inflammatory bowel diseases -- Periodicals; 616.344005
• DOI: 10.1093/ecco-jcc/jjw024
• Languages: English
• ISSNs: 1873-9946

Abstract

Abstract : Meta-omics [metagenomics, metatranscriptomics, and metaproteomics] are rapidly expanding our knowledge of the gut microbiota in health and disease. These technologies are increasingly used in inflammatory bowel disease [IBD] research. Yet, meta-omics data analysis, interpretation, and among-study comparison remain challenging. In this review we discuss the role these techniques are playing in IBD research, highlighting their strengths and limitations. We give guidelines on proper sample collection and preparation methods, and on performing the analyses and interpreting the results, reporting available user-friendly tools and pipelines.