A water soluble carbazolyl-BODIPY photosensitizer with an orthogonal D–A structure for photodynamic therapy in living cells and zebrafish. (2nd December 2019)

• Record Type: Journal Article
• Authors: Wang, Chengjun; Qian, Ying
• Is Part Of: Biomaterials science. Volume 8:Number 3(2020)
• Page Start: 830
• Page End: 836
• Publication Date: 2019-12-02
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/c9bm01709g
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : A photosensitizer Cz-BODIPY with an orthogonal D–A structure was developed and displayed promising potential in real PDT application. Abstract : A novel photosensitizer carbazolyl-BODIPY (Cz-BODIPY) with an orthogonal donor–acceptor structure was developed for photodynamic therapy (PDT). The photosensitizer Cz-BODIPY showed strong singlet oxygen sensitizing capability ( Φ Δ = 0.68 in MeOH), excellent water solubility in dilute solution, and high photostability. The photosensitizer Cz-BODIPY exhibited negligible dark cytotoxicity and high phototoxicity (IC50 0.45 μM). Cz-BODIPY could induce cell apoptosis upon light illumination. Three cell states including living cells, apoptotic cells, and dead cells in the PDT process of Cz-BODIPY were determined via the Hoechst 33342/PI dual staining assays. The ROS (reactive oxygen species) generation in living cells during the PDT process of Cz-BODIPY was captured by the ROS detector, dihydroethidium (DHE). The photosensitizer Cz-BODIPY could be assimilated by zebrafish to generate ROS and diminish the integrity of zebrafish tissue upon light illumination. Tumor cell growth could be inhibited by Cz-BODIPY upon light illumination. The photosensitizer Cz-BODIPY displayed potential in real PDT application.