Developing CRISPR‐based sex‐ratio distorters for the genetic control of fruit fly pests: A how to manual. Issue 3 (17th December 2019)

• Record Type: Journal Article
• Authors: Tsoumani, Konstantina T.; Meccariello, Angela; Mathiopoulos, Kostas D.; Papathanos, Philippos Aris
• Other Names: Toprak Umut guestEditor.
• Is Part Of: Archives of insect biochemistry and physiology. Volume 103:Issue 3(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-12-17
• Subjects: CRISPR/Cas9 -- Redkmer -- spermatogenesis -- X‐chromosome -- X‐shredding system; Insects -- Physiology -- Periodicals; Insect biochemistry -- Periodicals; 595.701572
• DOI: 10.1002/arch.21652
• Languages: English
• ISSNs: 0739-4462

Abstract

Abstract: Agricultural pest control using genetic‐based methods provides a species‐specific and environmentally harmless way for population suppression of fruit flies. One way to improve the efficiency of such methods is through self‐limiting, female‐eliminating approaches that can alter an insect populations' sex ratio toward males. In this microreview, we summarize recent advances in synthetic sex ratio distorters based on X‐chromosome shredding that can induce male‐biased progeny. We outline the basic principles to guide the efficient design of an X‐shredding system in an XY heterogametic fruit fly species of interest using CRISPR/Cas gene editing, newly developed computational tools, and insect genetic engineering. We also discuss technical aspects and challenges associated with the efficient transferability of this technology in fruit fly pest populations, toward the potential use of this new class of genetic control approaches for pest management purposes. Abstract : Design principles of the X‐shredding system in XY heterochromatic fruit flies using CRISPR/Cas nucleases, newly developed computational tools, and insect genetic engineering, toward the development of new genetic pest control approaches. Research Highlights: New genetic control approach in XY heterochromatic fruit flies Requirements for the generation of an X‐chromosome shredding system CRISPR‐based X‐shredders bias the sex ratio towards males, by targeting X‐specific repeats during spermatogenesis