Modulating the Biofunctionality of Metal–Organic‐Framework‐Encapsulated Enzymes through Controllable Embedding Patterns. Issue 7 (3rd January 2020)

• Record Type: Journal Article
• Authors: Chen, Guosheng; Kou, Xiaoxue; Huang, Siming; Tong, Linjing; Shen, Yujian; Zhu, Wangshu; Zhu, Fang; Ouyang, Gangfeng
• Is Part Of: Angewandte Chemie international edition. Volume 59:Issue 7(2020)
• Page Start: 2867
• Page End: 2874
• Publication Date: 2020-01-03
• Subjects: biocatalysis -- embedding patterns -- enzyme immobilization -- enzyme-surface modification -- metal–organic frameworks; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201913231
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Embedding an enzyme within a MOF as exoskeleton (enzyme@MOF) offers new opportunities to improve the inherent fragile nature of the enzyme, but also to impart novel biofunctionality to the MOF. Despite the remarkable stability achieved for MOF‐embedded enzymes, embedding patterns and conversion of the enzymatic biofunctionality after entrapment by a MOF have only received limited attention. Herein, we reveal how embedding patterns affect the bioactivity of an enzyme encapsulated in ZIF‐8. The enzyme@MOF can maintain high activity when the encapsulation process is driven by rapid enzyme‐triggered nucleation of ZIF‐8. When the encapsulation is driven by slow coprecipitation and the enzymes are not involved in the nucleation of ZIF‐8, enzyme@MOF tends to be inactive owing to unfolding and competing coordination caused by the ligand, 2‐methyl imidazole. These two embedding patterns can easily be controlled by chemical modification of the amino acids of the enzymes, modulating their biofunctionality. Abstract : Suit of armor : The effects of embedding patterns on the biofunctionality of MOF‐encapsulated enzymes are releaved. This work demostrates that the embedding patterns can easily be controlled through enzyme‐surface modification as a new strategy to construct enzymes@MOFs with excellent bioactivity and stability.