Functional Nanoparticles with a Reducible Tetrasulfide Motif to Upregulate mRNA Translation and Enhance Transfection in Hard‐to‐Transfect Cells. Issue 7 (9th January 2020)

• Record Type: Journal Article
• Authors: Wang, Yue; Tang, Jie; Yang, Yannan; Song, Hao; Fu, Jianye; Gu, Zhengying; Yu, Chengzhong
• Is Part Of: Angewandte Chemie international edition. Volume 59:Issue 7(2020)
• Page Start: 2695
• Page End: 2699
• Publication Date: 2020-01-09
• Subjects: mRNA delivery -- nanomaterials -- nanoregulators -- organosilica; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201914264
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Effective messenger RNA (mRNA) transfection in hard‐to‐transfect cells delivered by vectors is a long‐standing challenge. Now it is hypothesized that the high intracellular glutathione level is associated with suppressed mRNA translation. This theory leads to a new design principle of next‐generation mRNA vectors: nanoparticles with glutathione depletion chemistry upregulate mRNA translation and enhance transfection, which is beneficial for mRNA delivery in hard‐to‐transfect cells in vitro and in vivo. Abstract : My preciouSSSS : Conventional mRNA nano‐vectors limit their functions simply as transfection agents. Now, nanoparticles with a reducible tetrasulfide motif are presented that not only act as transfection agents, but also upregulate mRNA translation, thus achieving a higher in vitro and in vivo mRNA transfection efficacy in hard‐to‐transfect cells.