Changes in cardiac and hepatic energetic metabolism in gerbils infected by Listeria monocytogenes. (January 2020)

• Record Type: Journal Article
• Authors: Jaguezeski, Antonise M.; Souza, Carine F.; Perin, Géssica; Gebert, Roger R.; Baldi, Kelen R.A.; Gomes, Teane M.A.; Baldissera, Matheus D.; Andrade, Cinthia M.; Stefani, Lenita M.; Da Silva, Aleksandro S.
• Is Part Of: Microbial pathogenesis. Volume 138(2020)
• Publication Date: 2020-01
• Subjects: Oxidative stress -- Listeriosis -- Phosphotransferases -- ATPase; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2019.103786
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Energy metabolism is a sensitive indicator of cellular disorders. Therefore, the objective of this study was to investigate changes in cardiac and hepatic energy metabolism during listeriosis using an experimental model. We divided gerbils into two groups: Control (n = 11) and orally Infected (n = 12) with 5 × 10 9 CFU/mL of Listeria monocytogenes . Euthanasia and sampling were performed on days 6 and 12 post-infection (PI). Histopathological lesions were not found in the heart; however, the liver showed pyogranuloma. In the hearts of infected animals, cytosolic creatine kinase activity was lower on day 6 and 12 PI; mitochondrial creatine kinase/pyruvate kinase (PK), and sodium potassium pump (Na + /K + -ATPase) activities were lower on day 12 PI. Hepatic PK and Na + /K + -ATPase activities were lower in the infected group on day 12 PI. Lipoperoxidation was higher in the livers and hearts of infected animals on day 12 PI, and antioxidant capacity against peroxyl radicals (ACAP) was also higher in this group. These data suggest that subclinical listeriosis alters hepatic and cardiac energy metabolism, possibly related to decreased activity of phosphotransferases and ATPase. Subsequent antioxidant responses are not sufficient to correct alterations in lipid peroxidation and bioenergetics, possibly leading to important cellular pathological mechanisms. Graphical abstract: Image 1 Highlights: The CK-CIT and CK-MIT activity were lower in the heart of infected by L. monocytogenes. Reduction in activity of phosphotransferases and Na+/K + -ATPase were observed in heart and liver. Lipid peroxidation demonstrates a pronounced oxidizing response and helps to clarify the indirect damage of Na+/K + -ATPase action. ACAP levels were higher in the infected, but it was not able to neutralize lipoperoxidation.