Neopterin levels and Kyn/Trp ratios were significantly increased in dengue virus patients and subsequently decreased after recovery. (February 2020)

• Record Type: Journal Article
• Authors: Geisler, Simon; Lytton, Simon D.; Toan, Nguyen Linh; Nghia, Trinh Huu; Nam, Nguyen Minh; Hung, Hoang Vu; Son, Nguyen Thai; Anh, Do Tuan; Tuyen, Hoang Tien; Tien, Tran Viet; Quyet, Do; Van Tong, Hoang; Hoan, Nghiem Xuan; Song, Le Huu; Pallerla, Srinivas Reddy; Gostner, Johanna M.; Fuchs, Dietmar; Velavan, Thirumalaisamy P.
• Is Part Of: International journal of infectious diseases. Volume 91(2020)
• Page Start: 162
• Page End: 168
• Publication Date: 2020-02
• Subjects: Dengue -- Neopterin -- Tryptophan -- Kynurenine; Communicable diseases -- Periodicals; Communicable Diseases -- Periodicals; Communicable diseases; Periodicals; Electronic journals; 616.9
• DOI: 10.1016/j.ijid.2019.12.005
• Languages: English
• ISSNs: 1201-9712

Abstract

Highlights: Neopterin levels and Kyn/Trp ratios were significantly increased in dengue virus (DENV) patients and subsequently decreased after recovery. Neopterin concentrations strongly correlated with Kyn/Trp ratios. Neopterin levels and Kyn/Trp ratios could be used as indicators of the response to supportive care in DENV infection. Abstract: Objectives: During dengue fever, a pronounced gamma-interferon immune response produces neopterin and promotes tryptophan degradation by the enzyme indoleamine-2, 3-dioxygenase 1 (IDO-1). Activated IDO-1 is indicated by an increased kynurenine to tryptophan ratio (Kyn/Trp) in patients. Methods: Plasma levels of neopterin, kynurenine, and tryptophan were measured in 72 hospitalized dengue virus (DENV) patients and 100 healthy individuals. Plasma levels of neopterin, kynurenine, and tryptophan were also measured prospectively in a second cohort of 13 DENV patients; on the day of hospitalization, on day 2–3 at discharge, and 7–10 days after discharge. DENV RNA positivity was determined by qualitative and quantitative methodologies. Results: DENV RNA-positive patients presented significantly higher levels of neopterin (mean 36.5 nmol/l) and Kyn/Trp ratios (mean 102 μmol/mmol) compared to DENV RNA-negative individuals. A significant correlation between neopterin levels and Kyn/Trp ratios was observed in both DENV RNA-positive (Spearman's rho = 0.37, p < 0.01) and DENV RNA-negative (Spearman's rho = 0.89, p < 0.001) patients. Kyn/Trp ratios were negatively correlated with platelet counts (Spearman's rho = −0.43, p < 0.01) and positively correlated with liver enzymes: AST (Spearman's rho = 0.68, p < 0.01) and ALT (Spearman's rho = 0.51, p < 0.05). In addition, the follow-up data presented a significant decrease in neopterin levels and Kyn/Trp ratios within 10 days after hospital entry. Conclusions: Neopterin levels and Kyn/Trp ratios were significantly increased in DENV patients and subsequently decreased after recovery.