Flagellin peptide flg22 gains access to long-distance trafficking in Arabidopsis via its receptor, FLS2. (28th April 2017)

• Record Type: Journal Article
• Authors: Jelenska, Joanna; Davern, Sandra M.; Standaert, Robert F.; Mirzadeh, Saed; Greenberg, Jean T.
• Is Part Of: Journal of experimental botany. Volume 68:Number 7(2017)
• Page Start: 1769
• Page End: 1783
• Publication Date: 2017-04-28
• Subjects: Arabidopsis -- flagellin -- flg22 -- FLS2 -- long-distance traffic -- peptide -- Pseudomonas syringae -- receptor-mediated endocytosis -- transport.; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/erx060
• Languages: English
• ISSNs: 0022-0957

Abstract

Highlight: Receptor-mediated endocytosis enables flg22 internalization, trafficking to vacuoles, entry to vasculature and mobilization to distal tissues. Receptors can function similarly to transporters and allow uptake and long-distance transport of molecules. Abstract: Diverse pathogen-derived molecules, such as bacterial flagellin and its conserved peptide flg22, are recognized in plants via plasma membrane receptors and induce both local and systemic immune responses. The fate of such ligands was unknown: whether and by what mechanism(s) they enter plant cells and whether they are transported to distal tissues. We used biologically active fluorophore and radiolabeled peptides to establish that flg22 moves to distal organs with the closest vascular connections. Remarkably, entry into the plant cell via endocytosis together with the FLS2 receptor is needed for delivery to vascular tissue and long-distance transport of flg22. This contrasts with known routes of long distance transport of other non-cell-permeant molecules in plants, which require membrane-localized transporters for entry to vascular tissue. Thus, a plasma membrane receptor acts as a transporter to enable access of its ligand to distal trafficking routes.