Metabolic Labeling of Peptidoglycan with NIR‐II Dye Enables In Vivo Imaging of Gut Microbiota. (9th January 2020)

• Record Type: Journal Article
• Authors: Wang, Wei; Yang, Qinglai; Du, Yahui; Zhou, Xiaobo; Du, Xiaochen; Wu, Qiuyue; Lin, Liyuan; Song, Yanling; Li, Fuyou; Yang, Chaoyong; Tan, Weihong
• Is Part Of: Angewandte Chemie. Volume 132:Number 7(2020)
• Page Start: 2650
• Page End: 2655
• Publication Date: 2020-01-09
• Subjects: GI-Peristaltik -- Darmflora -- In-vivo-Imaging -- Mikrobielle Biogeographie -- NIR-II; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201910555
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Deepening our understanding of mammalian gut microbiota has been greatly hampered by the lack of a facile, real‐time, and in vivo bacterial imaging method. To address this unmet need in microbial visualization, we herein report the development of a second near‐infrared (NIR‐II)‐based method for in vivo imaging of gut bacteria. Using d ‐propargylglycine in gavage and then click reaction with an azide‐containing NIR‐II dye, gut microbiota of a donor mouse was strongly labeled with NIR‐II fluorescence on their peptidoglycan. The bacteria could be readily visualized in recipient mouse gut with high spatial resolution and deep tissue penetration under NIR irradiation. The NIR‐II‐based metabolic labeling strategy reported herein, provides, to the best of our knowledge, the first protocol for facile in vivo visualization of gut microbiota within deep tissues, and offers an instrumental tool for deciphering the complex biology of these gut "dark matters". Abstract : Darm mit Charme : Eine Echtzeit‐in‐vivo‐Bildgebungsmethode zur Visualisierung der Darmflora kombiniert d ‐Aminosäure‐basierte metabolische Markierung und NIR‐II‐Fluoreszenzbildgebung. Die Vielseitigkeit dieser Technik wurde durch Darstellung verschiedener Bakterienspezies sowie beim Studium gastrointestinaler motilitätsinduzierter Veränderungen der Biogeographie der Darmflora demonstriert.