Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation. Issue 1 (16th December 2019)

• Record Type: Journal Article
• Authors: Walsh, Sophie C.; Miles, Jeremy R.; Yao, Linxing; Broeckling, Corey D.; Rempel, Lea A.; Wright‐Johnson, Elane C.; Pannier, Angela K.
• Is Part Of: Molecular reproduction and development. Volume 87:Issue 1(2020)
• Page Start: 174
• Page End: 190
• Publication Date: 2019-12-16
• Subjects: blastocyst elongation -- metabolome -- porcine -- uterine environment; Reproduction -- Periodicals; Molecular biology -- Periodicals; Molecular genetics -- Periodicals; Embryology -- Periodicals; 571.8
• DOI: 10.1002/mrd.23306
• Languages: English
• ISSNs: 1040-452X

Abstract

Abstract: The objective of this study was to identify metabolites within the porcine uterine milieu during the early stages of blastocyst elongation. At Days 9, 10, or 11 of gestation, reproductive tracts of White cross‐bred gilts ( n = 38) were collected immediately following harvest and flushed with Roswell Park Memorial Institute‐1640 medium. Conceptus morphologies were assessed from each pregnancy and corresponding uterine flushings were assigned to one of five treatment groups based on these morphologies: (a) uniform spherical ( n = 8); (b) heterogeneous spherical and ovoid ( n = 8); (c) uniform ovoid ( n = 8); (d) heterogeneous ovoid and tubular ( n = 8); and (e) uniform tubular ( n = 6). Uterine flushings from these pregnancies were submitted for nontargeted profiling by gas chromatography–mass spectrometry (GC–MS) and ultra performance liquid chromatography (UPLC)–MS techniques. Unsupervised multivariate principal component analysis (PCA) was performed using pcaMethods and univariate analysis of variance was performed in R with false discovery rate (FDR) adjustment. PCA analysis of the GC–MS and UPLC–MS data identified 153 and 104 metabolites, respectively. After FDR adjustment of the GC–MS and UPLC–MS data, 38 and 59 metabolites, respectively, differed ( p < .05) in uterine flushings from pregnancies across the five conceptus stages. Some metabolites were greater ( p < .05) in abundance for uterine flushings containing earlier stage conceptuses (i.e., spherical), such as uric acid, tryptophan, and tyrosine. In contrast, some metabolites were greater ( p < .05) in abundance for uterine flushings containing later stage conceptuses (i.e., tubular), such as creatinine, serine, and urea. These data illustrate several putative metabolites that change within the uterine milieu during early porcine blastocyst elongation. Abstract : Alternations in the signaling of critical molecular factors within the uterine environment result in deficiencies in porcine blastocyst elongation, leading directly to embryonic loss as well as delayed elongation. This study illustrates several putative metabolites that change within the uterine environment as porcine conceptuses transition between spherical, ovoid, and tubular conceptuses. Variation of these metabolites in unique patterns provide possible molecular signals necessary to initiate and progress blastocyst thru the early stages of elongation.