Development of an LC–MS/MS method for quantifying two main metabolites of abivertinib in human plasma. (23rd December 2019)

• Record Type: Journal Article
• Authors: Zheng, Xin; Wang, Weicong; Zhang, Yanbao; Ma, Yuxiang; Zhao, Hongyun; Gao, Huitao; Hu, Pei; Jiang, Ji
• Is Part Of: Biomedical chromatography. Volume 34:Number 2(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-12-23
• Subjects: abivertinib -- human plasma -- metabolites -- pharmacokinetics -- UPLC‐MS/MS; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.4704
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: Abivertinib represents a highly selective irreversible epidermal growth factor receptor tyrosine kinase inhibitor. Two major metabolites of abivertinib, M7 and MII‐6, were detected in human plasma, which are recommended to be monitored for safety reasons in clinical trial. A high‐throughput quantification method utilizing liquid chromatography–tandem mass spectrometry was designed and verified to quantify abivertinib's primary metabolites in human plasma. Solid‐phase extraction was used to process the plasma, and then the analytes underwent a gradient elution separation in an Aquity UPLC BEH C18 column (1.7 μm, 2.1 × 50 mm) with mobile phase A (10 mm ammonium acetate containing 0.1% formic acid) and mobile phase B (methanol–acetonitrile, 2:8, v/v, with 0.1% formic acid). Ion transitions of M7 ( m / z 490.2 → 405.1) and MII‐6 ( m / z 476.2 → 391.1) were monitored under multiple reaction monitoring mode and electrospray ionization in positive ion mode. This simultaneous determination method was found to have acceptable precision, accuracy and linearity in the 0.5–500 ng/mL range for M7 and the 0.5–500 ng/mL range for MII‐6, accompanied by a mild matrix effect but high recovery. Further stability assessments indicated that both analytes remained stable throughout the entire experimental process from harvesting whole blood to plasma extraction and analysis.