Real‐Time NMR Spectroscopy for Studying Metabolism. (20th December 2019)

• Record Type: Journal Article
• Authors: Alshamleh, Islam; Krause, Nina; Richter, Christian; Kurrle, Nina; Serve, Hubert; Günther, Ulrich L.; Schwalbe, Harald
• Is Part Of: Angewandte Chemie. Volume 132:Number 6(2020)
• Page Start: 2324
• Page End: 2328
• Publication Date: 2019-12-20
• Subjects: Biochemie -- Echtzeit-NMR-Spektroskopie -- Personalisierte Medizin -- Stoffwechsel -- Zellstudien; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201912919
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Current metabolomics approaches utilize cellular metabolite extracts, are destructive, and require high cell numbers. We introduce here an approach that enables the monitoring of cellular metabolism at lower cell numbers by observing the consumption/production of different metabolites over several kinetic data points of up to 48 hours. Our approach does not influence cellular viability, as we optimized the cellular matrix in comparison to other materials used in a variety of in‐cell NMR spectroscopy experiments. We are able to monitor real‐time metabolism of primary patient cells, which are extremely sensitive to external stress. Measurements are set up in an interleaved manner with short acquisition times (approximately 7 minutes per sample), which allows the monitoring of up to 15 patient samples simultaneously. Further, we implemented our approach for performing tracer‐based assays. Our approach will be important not only in the metabolomics fields, but also in individualized diagnostics. Abstract : Klare Sicht auf die Dinge : Ein neu entwickelter Ansatz zur Überwachung des Stoffwechsels von Lebendzellen in einer zellfreundlichen Umgebung wird vorgestellt und rückt somit die NMR‐Spektroskopie näher an personalisierte Medizin.