RNU6-1 in circulating exosomes differentiates GBM from non-neoplastic brain lesions and PCNSL but not from brain metastases. Issue 2 (31st January 2020)

• Record Type: Journal Article
• Authors: Puigdelloses, Montse; González-Huárriz, Marisol; García-Moure, Marc; Martínez-Vélez, Naiara; Esparragosa Vázquez, Inés; Bruna, Jordi; Zandio, Beatriz; Agirre, Amaia; Marigil, Miguel; Petrirena, Gregorio; Nuñez-Córdoba, Jorge M; Tejada-Solís, Sonia; Díez-Valle, Ricardo; Gállego-Culleré, Jaime; Martínez-Vila, Eduardo; Patiño-García, Ana; Alonso, Marta M; Gállego Pérez-Larraya, Jaime
• Is Part Of: Neuro-oncology advances. Volume 2:Issue 2(2020)
• Publication Date: 2020-01-31
• Subjects: Glioblastoma -- biomarkers -- serum -- exosomes -- RNU6-1 -- miRNA; 616.99481
• DOI: 10.1093/noajnl/vdaa010
• Languages: English
• ISSNs: 2632-2498

Abstract

Abstract: Background: Glioblastoma (GBM) is the most common malignant primary brain tumour in adults. Circulating biomarkers may assist in the processes of differential diagnosis and response assessment. GBM cells release extracellular vesicles containing a subset of proteins and nucleic acids. We previously demonstrated that exosomes isolated from the serum of GBM patients had an increased expression of RNU6-1 compared to healthy subjects. In this exploratory study we investigated the role of this small non-coding RNA as a diagnostic biomarker for GBM versus other brain lesions with some potential radiological similarities. Methods: We analysed the expression of RNU6-1 in circulating exosomes of GBM patients (n=18), healthy controls (n=30), and patients with subacute stroke (n=30), acute/subacute haemorrhage (n=30), acute demyelinating lesions (n=18), brain metastases (n=21) and primary central nervous system lymphoma (PCNSL; n=12) using ddPCR. Results: Expression of RNU6-1 was significantly higher in GBM patients than in healthy controls (p=0.002). RNU6-1 levels were also significantly higher in exosomes from GBM patients than from patients with non-neoplastic lesions (stroke [p=0, 05], haemorrhage [p=0.01], demyelinating lesions [p=0.019]) and PCNSL (p=0.004). In contrast, no significant differences were found between patients with GBM and brain metastases (p=0.573). ROC curve analyses supported the role of this biomarker in differentiating GBM from subacute stroke, acute/subacute haemorrhage, acute demyelinating lesions and PCNSL (p<0.05), but again not from brain metastases (p=0.575). Conclusions: Our data suggest that the expression of RNU6-1 in circulating exosomes could be useful for the differentiation of GBM from non-neoplastic brain lesions and PCNSL, but not from brain metastases.