Local surface plasmon resonance of gold nanoparticles as a correlative light and electron microscopy (CLEM) tag for biological samples. (28th October 2019)

• Record Type: Journal Article
• Authors: Haruta, Tomohiro; Hasumi, Keiko; Ikeda, Yuta; Konyuba, Yuji; Fukuda, Tomohisa; Nishioka, Hideo
• Is Part Of: Microscopy. Volume 68:Number 6(2019)
• Page Start: 467
• Page End: 470
• Publication Date: 2019-10-28
• Subjects: correlative microscopy -- SiN film -- LSPR -- Au NPs -- imaging tag; Microscopy -- Periodicals; 502.825
• DOI: 10.1093/jmicro/dfz031
• Languages: English
• ISSNs: 2050-5698

Abstract

Abstract: In this study, we investigated use of local surface plasmon resonance (LSPR) of metal nanoparticles (NPs) as a correlative light and electron microscopy (CLEM) tag for biological samples. Gold NPs in ultra-thin sections for TEM revealed that LSPR could be observed by optical microscopy at sizes of 20 nm or larger. Gold NPs at sizes less than 20 nm could be observed using the gold enhancement method. Therefore, this CLEM tag could be applied to immunoelectron microscopy using this gold enhancement method.