Advanced strategy for metabolite exploration in filamentous fungi. (17th February 2020)

• Record Type: Journal Article
• Authors: Deng, Huaxiang; Bai, Yajun; Fan, Tai-Ping; Zheng, Xiaohui; Cai, Yujie
• Is Part Of: Critical reviews in biotechnology. Volume 40:Number 2(2020)
• Page Start: 180
• Page End: 198
• Publication Date: 2020-02-17
• Subjects: Natural products -- filamentous fungi -- high throughput engineering -- pathway deconstruction -- drug discovery -- biosynthetic gene clusters -- metabolic balance; Biotechnology -- Periodicals; Biotechnology -- Periodicals; Review Literature -- Periodicals; Public Health -- Periodicals; Environment -- Periodicals; Industry -- Periodicals; Biotechnology; Review Literature; Public Health; Environment; Industry; 660.6
• DOI: 10.1080/07388551.2019.1709798
• Languages: English
• ISSNs: 0738-8551

Abstract

Abstract: Filamentous fungi comprise an abundance of gene clusters that encode high-value metabolites, whereas affluent gene clusters remain silent during laboratory conditions. Complex cellular metabolism further limits these metabolite yields. Therefore, diverse strategies such as genetic engineering and chemical mutagenesis have been developed to activate these cryptic pathways and improve metabolite productivity. However, lower efficiencies of gene modifications and screen tools delayed the above processes. To address the above issues, this review describes an alternative design-construction evaluation optimization (DCEO) approach. The DCEO tool provides theoretical and practical principles to identify potential pathways, modify endogenous pathways, integrate exogenous pathways, and exploit novel pathways for their diverse metabolites and desirable productivities. This DCEO method also offers different tactics to balance the cellular metabolisms, facilitate the genetic engineering, and exploit the scalable metabolites in filamentous fungi.