A rigorous exploration of anal HPV genotypes using a next‐generation sequencing (NGS) approach in HIV‐infected men who have sex with men at risk for developing anal cancer. (25th November 2019)

• Record Type: Journal Article
• Authors: Piyathilake, Chandrika J.; Badiga, Suguna; Kumar, Ranjit; Crowley, Michael R.; Burkholder, Greer A.; Raper, James L.
• Is Part Of: Cancer medicine. Volume 9:Number 2(2020)
• Page Start: 807
• Page End: 815
• Publication Date: 2019-11-25
• Subjects: anal cytology -- HIV -- HPV -- MSM -- next‐generation sequencing; 616.994005
• DOI: 10.1002/cam4.2720
• Languages: English
• ISSNs: 2045-7634

Abstract

Abstract: Background: There are no HPV‐based measures for managing anal cancer (AC) in HIV‐infected (HIV+) men who have sex with men (MSM) because of the high positivity of high‐risk (HR)‐HPVs. As next‐generation sequencing (NGS) is able to describe the composition of HPVs as percent (%) reads rather than positive vs negative results, we used NGS approach to detect HPVs in anal samples of HIV+ MSM to test its ability to differentiate those who are diagnosed with atypical squamous cells of unknown significance or greater (ASCUS+) from those who are free of such lesions and to understand the burden of HPV infections in relation to HPV vaccines. Methods: Study included 81 HIV+ MSM characterized for demographics, patient‐reported outcome measures, HIV related laboratory measures and anal cytology. We summarized NGS HPV data using % read cut points (>0%‐>30%) and tested the relationship between % reads of HR‐HPVs and risk of ASCUS+ using logistic regression. Results: Forty‐six HPVs were detected at the >0% read cut point. The prevalence of any HR‐HPVs varied from 100% to 40% with >0% to >30% reads while ≥99% were infected with HR‐HPVs included or not included in the 9 valent HPV vaccine at the >0% read cut point. MSM with >30% HR‐HPV reads were 4.5 times more likely to be diagnosed with ASCUS+ compared to ≤30% reads ( P = .033). Conclusion: NGS‐based approach is more accurate than PCR‐based HPV testing for identifying HIV+ MSM at risk for developing AC. We raise the concern regarding the efficacy of current HPV vaccines for preventing AC in this high‐risk population. Abstract : Next‐generation sequencing‐based approach is more accurate than PCR‐based HPV testing for identifying HIV+ MSM at risk for developing anal cancer. We raise the concern regarding the efficacy of current HPV vaccines for preventing anal cancer in this high‐risk population.