Biomimetic Elastomeric Bioactive Siloxane‐Based Hybrid Nanofibrous Scaffolds with miRNA Activation: A Joint Physico‐Chemical‐Biological Strategy for Promoting Bone Regeneration. (7th November 2019)

• Record Type: Journal Article
• Authors: Yu, Meng; Du, Yuzhang; Han, Yong; Lei, Bo
• Is Part Of: Advanced functional materials. Volume 30:Number 4(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-11-07
• Subjects: bioactive scaffolds -- bone tissue engineering -- siloxane‐based biomaterials -- sustained miRNA release; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.201906013
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract: Rapid and efficient disease‐induced or critical‐size bone regeneration remains a challenge in tissue engineering due to the lack of highly bioactive biomaterial scaffolds. Physical structures such as nanostructures, chemical components such as silicon elements, and biological factors such as genes have shown positive effects on bone regeneration. Herein, a bioactive photoluminescent elastomeric silicate‐based nanofibrous scaffold with sustained miRNA release is reported for promoting bone regeneration based on a joint physico‐chemical‐biological strategy. Bioactive nanofibrous scaffolds are fabricated by cospinning poly (ε‐caprolactone) (PCL), elastomeric poly (citrates‐siloxane) (PCS), and bioactive osteogenic miRNA nanocomplexes (denoted PPM nanofibrous scaffolds). The PPM scaffolds possess uniform nanostructures, significantly enhanced tensile stress (≈15 MPa) and modulus (≈32 MPa), improved hydrophilicity (30–60°), controlled biodegradation, and strong blue fluorescence. Bioactive miRNA complexes are efficiently loaded into the nanofibrous matrix and exhibit long‐term release for up to 70 h. The PPM scaffolds significantly promote the adhesion, proliferation, and osteoblast differentiation of bone marrow stem cells in vitro and enhanced rat cranial defect restoration (12 weeks) in vivo. This work reports an attractive joint physico‐chemical‐biological strategy for the design of novel cell/protein‐free bioactive scaffolds for synergistic tissue regeneration. Abstract : This paper reports a joint physico‐chemical‐biological strategy for synergistic bone tissue regeneration. A bioactive photoluminescent elastomeric siloxane‐based hybrid nanofibrous scaffold with sustained long‐term miRNA release is developed. The hybrid nanofibrous scaffolds significantly promote the adhesion, proliferation, and osteoblast differentiation of bone marrow stem cells in vitro and enhanced rat cranial defect restoration and bone tissue regeneration (12 weeks) in vivo.