Copaifera langsdorffii Desf.: in vitro investigation on anti-Helicobacter pylori and anti-inflammatory activities of oleoresin and fruit methanolic extract. (2nd January 2020)

• Record Type: Journal Article
• Authors: Governa, Paolo; Biagi, Marco
• Is Part Of: Plant biosystems. Volume 154:Number 1(2020)
• Page Start: 117
• Page End: 124
• Publication Date: 2020-01-02
• Subjects: Copaifera langsdorffii Desf. -- copaiba -- Helicobacter pylori -- IL-6 -- gastritis -- ethnopharmacology -- medicinal plants -- antimicrobial; Botany -- Periodicals; Biological systems -- Periodicals; Plants -- Periodicals; 581
• DOI: 10.1080/11263504.2019.1578284
• Languages: English
• ISSNs: 1126-3504

Abstract

Abstract: Copaifera langsdorffii Desf. has been used in the traditional medicine of Brazil for centuries to treat a wide range of conditions, including infections, wounds and inflammation. The aim of this study is to compare the effects of C. langsdorffii bark oleoresin and a methanolic extract of the fruit for their anti- Helicobacter pylori (HP) activity and gastroprotective effects. We analyzed the in vitro anti-HP activity against three HP strains: CCUG 39500 (cagA+) and two clinical isolates, G21 and 10 K (cagA + and cagA–, respectively). The effect of C. langsdorffii products on IL-6 release in lipopolysaccharide-stimulated human peripheral blood mononuclear cells was also evaluated. Finally, the toxicity of the samples was evaluated in vitro and in vivo. Both the samples showed an interesting anti-HP activity, acting on different virulence strains, with the oleoresin reaching a minimum bactericidal concentration of 98 μg/ml against cagA– strain. Moreover, an interesting reduction in IL-6 release was observed, which could be helpful for reducing HP-induced inflammation, thus, preventing complications such as ulcer and irritation of the gastric mucosa. Toxicity was not observed up to 2000 µg/ml. Even if more studies must be conducted, this work supports the use of C. langsdorffii in the management of HP-related gastric disorders.