Tryptophan promoted β-defensin-2 expression via the mTOR pathway and its metabolites: kynurenine banding to aryl hydrocarbon receptor in rat intestine. Issue 6 (21st January 2020)

• Record Type: Journal Article
• Authors: Tang, Zhiru; Shi, Baoshi; Sun, Weizhong; Yin, Yulong; Chen, Qingju; Mohamed, Taha; Lu, Changwen; Sun, Zhihong
• Is Part Of: RSC advances. Volume 10:Issue 6(2020)
• Page Start: 3371
• Page End: 3379
• Publication Date: 2020-01-21
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c9ra10477a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : In this study, we investigated the signalling pathways mediating tryptophan (Trp)-promoted β-defensin-2 (BD-2) expression in rat intestinal mucosa. Abstract : In this study, we investigated the signalling pathways mediating tryptophan (Trp)-promoted β-defensin-2 (BD-2) expression in rat intestinal mucosa. Sprague Dawley rats were administered with l -Trp and treated with rapamycin (RAPA), 1-methyltryptophan (1-MT), or para -chlorophenyl-amine (PCPA) to inhibit mammalian target of rapamycin (mTOR), indoleamine-2, 3-dioxygenase (IDO), or tryptophan hydroxylase (TPH), respectively. The mRNA and protein levels of BD-2 in the jejunal and ileal mucosa of rats increased with administration of l -Trp. Intraperitoneal injection of RAPA significantly decreased the mRNA level of BD-2 and the concentrations of p -mTORC1 and BD-2 in the jejunal and ileal mucosa of rats with administration of l -Trp ( P < 0.05). Oral administration of 1-MT decreased the IDO activity and the mRNA and protein levels of BD-2, and increased the concentrations of tumour necrosis factor (TNF-α), interleukin (IL)-17, and IL-22 in the jejunal and ileal mucosa of rats with administration of l -Trp ( P < 0.05). Intraperitoneal injection of PCPA decreased the TPH activity and increased the mRNA and protein levels of BD-2, but did not change the concentrations of TNF-α, IL-17, or IL-22 in the jejunal and ileal mucosa of rats with administration of l -Trp. The results indicate the Trp-promoted BD-2 expression in the jejunum and ileum via the mTOR pathway and its metabolites: kynurenine banding to aryl hydrocarbon receptor in rat intestine.