'There is only one thing that is truly important in an IVF laboratory: everything' Cairo Consensus Guidelines on IVF Culture Conditions. Issue 1 (January 2020)

• Record Type: Journal Article
• Authors: Consensus Group, Cairo
• Is Part Of: Reproductive biomedicine online. Volume 40:Issue 1(2020)
• Page Start: 33
• Page End: 60
• Publication Date: 2020-01
• Subjects: Culture conditions -- Incubators -- IVF -- Maintenance -- Medium -- Quality control; Human reproductive technology -- Periodicals; Human embryo -- Periodicals; Reproduction -- Periodicals; 616.692
• DOI: 10.1016/j.rbmo.2019.10.003
• Languages: English
• ISSNs: 1472-6483

Abstract

Abstract: This proceedings report presents the outcomes from an international expert meeting to establish consensus guidelines on IVF culture conditions. Topics reviewed and discussed were: embryo culture – basic principles and interactions; temperature in the IVF laboratory; humidity in culture; carbon dioxide control and medium pH; oxygen tension for embryo culture; workstations – design and engineering; incubators – maintaining the culture environment; micromanipulation – maintaining a steady physcochemical environment; handling practices; assessment practices; culture media – buffering and pH, general composition and protein supplementation, sequential or single-step media for human embryo culture; use and management – cold chain and storage; test equipment – calibration and certification; and laboratory equipment and real-time monitoring. More than 50 consensus guideline points were established under these general headings.