Exogenous application of abscisic acid to shoots promotes primary root cell division and elongation. (March 2020)

• Record Type: Journal Article
• Authors: Xie, Qijun; Essemine, Jemaa; Pang, Xiaochen; Chen, Haiying; Cai, Weiming
• Is Part Of: Plant science. Volume 292(2020)
• Publication Date: 2020-03
• Subjects: ABA abscisic acid -- ABACUS abscisic acid concentration and uptake sensor -- GUS β-glucuronidase -- EZ elongation zone -- FRET fluorescent resonance energy transfer -- GFP green fluorescent protein -- HPTS 8-hydroxypyrene-13, 6-trisulfonic acid trisodium salt -- IAA indole-3-acetic acid -- MS Murashige and Skoog -- PRs primary roots -- PIN1 PIN-FORMED1 -- PI propidium iodide -- TIBA 2, 3, 5-triiodobenzoic acid -- YFP yellow fluorescent protein; Primary root -- ABA -- Cell division -- Auxin -- Basipetal -- Cell wall alkalinization; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2019.110385
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Proved the existence of basipetal regulatory effect of shoot derived ABA on primary roots growth. Involvement of hormonal crosstalk in transducing ABA signals from shoot to root. Alkalization promoted cell elongation stands a supplement to the long standing "acid growth theory". Confocal technology enabled deep investigation into the mechanism of shoot-ABA promoted primary root growth. Abstract: Root-derived abscisic acid (ABA) is known to regulate shoot physiology, such as stomata closure. Conversely, the basipetal regulatory effect of shoot-derived ABA is poorly understood. Herein, we report that simulation of shoot-ABA accumulation by exogenous application of ABA to shoots basipetally stimulates primary root (PR) growth. ABA applied to shoots accelerates root cell division, as evidenced by the increase in meristem size and cell number and the intensity of CYCB1;1::GFP (a mitosis marker). Root ABA content was not changed following shoot ABA application, although the ABA reporter line RAB18::GFP showed an increase in ABA in the cotyledons. Shoot-ABA application increases basipetal auxin transport by 114 %. Shoot-ABA-promoted PR growth can be abolished by attenuating basipetal auxin flux using 2, 3, 5-triiodobenzoic acid (TIBA, an auxin transport inhibitor), demonstrating that ABA promotes PR growth by increasing basipetal auxin transport. Root cell elongation, evaluated by the total length of the first 7 cells in the elongation zone (EZ), was increased by 56 % following shoot-ABA application. The cell walls of the root EZ were alkalinized by ABA, as exhibited by 8-hydroxypyrene-1, 3, 6-trisulfonic acid trisodium salt staining. Higher pH promotes both PR growth and cell elongation. Thus, shoot-ABA promotes cell elongation by alkalinizing the cell wall. In light of our results, we provide a representative detailed model of the basipetal regulatory effect of ABA on PR growth.