Site-directed RNA editing (SDRE): Off-target effects and their countermeasures. (20th November 2019)

• Record Type: Journal Article
• Authors: Mao, Shaoshuai; Liu, Yajing; Huang, Shisheng; Huang, Xingxu; Chi, Tian
• Is Part Of: Journal of genetics and genomics. Volume 46:Number 11(2019)
• Page Start: 531
• Page End: 535
• Publication Date: 2019-11-20
• Subjects: Gene editing -- RNA editing -- Cas13 -- Off-target -- Specificity; Genetics -- Periodicals; Genomics -- Periodicals; 576.505
• DOI: 10.1016/j.jgg.2019.11.005
• Languages: English
• ISSNs: 1673-8527

Abstract

Abstract: Site-directed RNA editing (SDRE) is invaluable to basic research and clinical applications and has emerged as a new frontier in genome editing. The past few years have witnessed a surge of interest in SDRE, with SDRE tools emerging at a breathtaking pace. However, off-target effects of SDRE remain a tough problem, which constitutes a major hurdle to their clinical applications. Here we discuss the diverse strategies for combating off-target editing, drawing lessons from the published studies as well as our ongoing research. Overall, SDRE is still at its infancy, with significant challenges and exciting opportunities ahead.