Boosting performance of self-powered biosensing device with high-energy enzyme biofuel cells and cruciform DNA. (February 2020)

• Record Type: Journal Article
• Authors: Wang, Fu-Ting; Wang, Yi-Han; Xu, Jing; Huang, Ke-Jing; Liu, Zhen-hua; Lu, Yun-fei; Wang, Shu-yu; Han, Zi-wei
• Is Part Of: Nano energy. Volume 68(2020)
• Publication Date: 2020-02
• Subjects: Enzymatic biofuel cell -- Self-powered biosensors -- MicroRNA -- Cruciform DNA -- Signal amplification; Nanoscience -- Periodicals; Nanotechnology -- Periodicals; Nanostructured materials -- Periodicals; Power resources -- Technological innovations -- Periodicals; Nanoscience; Nanostructured materials; Nanotechnology; Power resources -- Technological innovations; Periodicals; 621.042
• DOI: 10.1016/j.nanoen.2019.104310
• Languages: English
• ISSNs: 2211-2855

Abstract

Abstract: A self-powered microRNA (miRNA) biosensing device is fabricated with high-energy enzymatic biofuel cell (EBFC) and cruciform DNA (cDNA). Sulfur-selenium co-doped graphene/gold nanoparticles (S–Se-GR/AuNPs) is synthesized and used as supporting substrate of biocathode and bioanode. The glucose oxidase (GOD) molecules are bonded to carboxyl-functionalized AuNPs through the condensation reaction between the amino groups in enzyme and carboxyl groups on the AuNPs to form the bioanode. The ultra-thin porous carbon shell/AuNPs-complementary strand of cDNA (UPCS/AuNPs-cDNA) is meticulously designed. The potassium ferricyanides are then inserted in mesoporous UPCS/AuNPs as the biocathode electron acceptor, and the cruciform DNA bioconjugate is synthesized as signal amplifier. When target miRNA is added, the hybridization reaction happens between miRNA and capture probe DNA on the biocathode to open the capture probe DNA chain. Cruciform DNA bioconjugate is immobilized onto the biocathode through base pairing with the capture DNA on the biocathode, which can release electron acceptor [Fe(CN)6 ] 3-, resulting in the dramatically increase of the open circuit voltage of the EBFCs. The self-powered biosensor responds linearly in the miRNA level range of 0.5–10000 fM with a detection limit of 1.5 × 10 −16 mol L −1 . Detection of miRNA in spiked serum samples is also realized with the self-powered biosensor, demonstrating its great potential in the clinical applications. Graphical abstract: Image 1 Highlights: A self-powered biosensor is fabricated based on high-energy enzymatic biofuel cell and cruciform DNA. Sulfur-selenium co-doped graphene/gold nanoparticles is prepared and used for supporting substrate. [Fe(CN)6 ] 3- is entrapped in the UPCS/AuNPs by using cruciform DNA bioconjugate signal amplification. The self-powered biosensor shows a detection limit of 0.15 fM and high specificity towards target microRNA.