Effects of Bacillus licheniformis on the growth, antioxidant capacity, intestinal barrier and disease resistance of grass carp (Ctenopharyngodon idella). Issue 97 (February 2020)

• Record Type: Journal Article
• Authors: Qin, Lu; Xiang, Jinhua; Xiong, Fan; Wang, Guitang; Zou, Hong; Li, Wenxiang; Li, Ming; Wu, Shangong
• Is Part Of: Fish & shellfish immunology. Issue 97(2020)
• Page Start: 344
• Page End: 350
• Publication Date: 2020-02
• Subjects: Bacillus licheniformis -- Grass carp -- Ctenopharyngodon idella -- Growth -- Antioxidant capacity -- Intestinal barrier -- Disease resistance; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.12.040
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: To study the effect of dietary supplementation of Bacillus licheniformis FA6 on the growth, survival and intestinal health of grass carp, we assessed the antioxidant capacity, intestinal barrier, expression levels of immune genes, and the resistance to Aeromonas hydrophila AH-1 infection. Experimental setup comprised three groups (90 specimens each; average initial weight = 16.5 g): the control group was fed the basal diet without B. licheniformis, the low-dose (LD) group was supplemented with B. licheniformis at the concentration of 1 × 10 5 cfu/g, and the high-dose (HD) group with 1 × 10 6 cfu/g. After 56 days of growth trial, the challenge test with A. hydrophila AH-1 was conducted for 14 days. The results revealed that the grass carp in LD group and HD group had significantly ( p < 0.05) improved percent weight gain (PWG) and specific growth rate (SGR) parameters. Additionally, the antioxidant status was improved, which included increased superoxide dismutase (SOD) activity and decreased malondialdehyde (MDA) levels in the serum, and upregulated mRNA levels of antioxidant enzymes MnSOD and catalase (CAT) in the intestine. Meanwhile, B. licheniformis FA6 supplementation groups exhibited a decreased mRNA expression of proinflammatory cytokines (such as IL-1β and TNF-α ) and increased the expression of anti-inflammatory cytokine IL-10. Histological (villi length was increased) and gene expression (qPCR: upregulated ZO-1, occludin, and claudin-c ) analyses suggested improved functioning of the intestinal barrier. Post-challenge mortality rates in LD and HD groups were significantly lower (56.6% and 70% respectively) than in the control group (100%). Overall, these results indicated that dietary supplementation of B. licheniformis FA6 can improve growth, antioxidant capacity, intestinal barrier functions and disease resistance of grass carp. Highlights: Supplementing Bacillus licheniformis FA6 enhanced growth performance of grass carp. …increased the antioxidant capacity of grass carp. …strengthened the tight junctions and down-regulated pro-inflammatory cytokines in the intestine. …and enhanced its resistance against Aeromonas hydrophila.