Sp-CBL inhibits white spot syndrome virus replication by enhancing apoptosis in mud crab (Scylla paramamosain). (April 2020)

• Record Type: Journal Article
• Authors: Kong, Tongtong; Lin, Shanmeng; Gong, Yi; Tran, Ngoc Tuan; Zhang, Yueling; Zheng, Huaiping; Ma, Hongyu; Li, Shengkang
• Is Part Of: Developmental and comparative immunology. Volume 105(2020)
• Publication Date: 2020-04
• Subjects: Scylla paramamosain -- Casitas B-Lineage lymphoma -- Caspase -- Apoptosis; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2019.103580
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: In mammals, casitas B-lineage lymphoma (CBL) family proteins, a RING-type E3 ubiquitin ligase, are involved in many signal transduction pathways. However, the functions of CBL in invertebrates are not well elucidated. In this study, Sp -CBL containing CBL-N, CBL-2, CBL-3 and RING domains was identified in mud crab Scylla paramamosain . Sp -CBL was widely expressed in all tissues tested and found to be significantly up-regulated in the hemocytes of mud crab challenged by white spot syndrome virus (WSSV). The RNA interference of Sp -CBL increased the copy number of WSSV and declined the apoptosis rate of hemocytes. In addition, Sp -CBL could affect the activities of caspase 3 and the mitochondrial membrane potential. Taken together, the results of this study revealed that Sp -CBL could restrict WSSV proliferation through enhancing the apoptosis of the hemocytes, which would provide a novel insight into the anti-viral response in the innate immunity system of mud crab. Highlights: Sp -CBL was significantly up-regulated in the hemocytes of mud crab upon WSSV challenge. Sp -CBL could affect the activities of caspase 3 and the mitochondrial membrane potential. Sp -CBL restricts WSSV proliferation through enhancing the apoptosis of the hemocytes.