Enhanced processivity of Dnmt1 by monoubiquitinated histone H3. (3rd December 2019)
- Record Type:
- Journal Article
- Title:
- Enhanced processivity of Dnmt1 by monoubiquitinated histone H3. (3rd December 2019)
- Main Title:
- Enhanced processivity of Dnmt1 by monoubiquitinated histone H3
- Authors:
- Mishima, Yuichi
Brueckner, Laura
Takahashi, Saori
Kawakami, Toru
Otani, Junji
Shinohara, Akira
Takeshita, Kohei
Garvilles, Ronald Garingalao
Watanabe, Mikio
Sakai, Norio
Takeshima, Hideyuki
Nachtegael, Charlotte
Nishiyama, Atsuya
Nakanishi, Makoto
Arita, Kyohei
Nakashima, Kinichi
Hojo, Hironobu
Suetake, Isao - Abstract:
- Abstract: DNA methylation controls gene expression, and once established, DNA methylation patterns are faithfully copied during DNA replication by the maintenance DNA methyltransferase Dnmt1. In vivo, Dnmt1 interacts with Uhrf1, which recognizes hemimethylated CpGs. Recently, we reported that Uhrf1‐catalyzed K18‐ and K23‐ubiquitinated histone H3 binds to the N‐terminal region (the replication focus targeting sequence, RFTS) of Dnmt1 to stimulate its methyltransferase activity. However, it is not yet fully understood how ubiquitinated histone H3 stimulates Dnmt1 activity. Here, we show that monoubiquitinated histone H3 stimulates Dnmt1 activity toward DNA with multiple hemimethylated CpGs but not toward DNA with only a single hemimethylated CpG, suggesting an influence of ubiquitination on the processivity of Dnmt1. The Dnmt1 activity stimulated by monoubiquitinated histone H3 was additively enhanced by the Uhrf1 SRA domain, which also binds to RFTS. Thus, Dnmt1 activity is regulated by catalysis (ubiquitination)‐dependent and ‐independent functions of Uhrf1. Abstract : Monoubiquitinated histone H3 stimulates the processivity of Dnmt1. Dnmt1 activity stimulated by monoubiquitinated histone H3 is additively enhanced by the Uhrf1 SRA domain.
- Is Part Of:
- Genes to cells. Volume 25:Number 1(2020)
- Journal:
- Genes to cells
- Issue:
- Volume 25:Number 1(2020)
- Issue Display:
- Volume 25, Issue 1 (2020)
- Year:
- 2020
- Volume:
- 25
- Issue:
- 1
- Issue Sort Value:
- 2020-0025-0001-0000
- Page Start:
- 22
- Page End:
- 32
- Publication Date:
- 2019-12-03
- Subjects:
- Dnmt1 -- methylation -- processivity -- RFTS -- SRA -- ubiquitinated histone
Cytogenetics -- Periodicals
Cells -- Mechanical properties -- Periodicals
Molecular genetics -- Periodicals
Genes -- Periodicals
Molecular biology -- Periodicals
Cytology -- Periodicals
Biomechanics -- Periodicals
571.6 - Journal URLs:
- http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2443 ↗
http://www.blacksci.co.uk/%7Ecgilib/jnlpage.bin?Journal=GTC&File=GTC&Page=aims ↗
http://onlinelibrary.wiley.com/ ↗ - DOI:
- 10.1111/gtc.12732 ↗
- Languages:
- English
- ISSNs:
- 1356-9597
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 4111.762500
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 12618.xml