An overview on enrichment methods for cell surface proteome profiling. Issue 1 (30th September 2019)

• Record Type: Journal Article
• Authors: Li, Yanan; Qin, Hongqiang; Ye, Mingliang
• Other Names: Liu Huwei guestEditor.
• Is Part Of: Journal of separation science. Volume 43:Issue 1(2020)
• Page Start: 292
• Page End: 312
• Publication Date: 2019-09-30
• Subjects: cell surface proteins -- enrichment methods -- plasma membrane proteins -- proteomics; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201900700
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract: Cell surface proteins are essential for many important biological processes, including cell–cell interactions, signal transduction, and molecular transportation. With the characteristics of low abundance, high hydrophobicity, and high heterogeneity, it is difficult to get a comprehensive view of cell surface proteome by direct analysis. Thus, it is important to selectively enrich the cell surface proteins before liquid chromatography with mass spectrometry analysis. In recent years, a variety of enrichment methods have been developed. Based on the separation mechanism, these methods could be mainly classified into three types. The first type is based on their difference in the physicochemical property, such as size, density, charge, and hydrophobicity. The second one is based on the bimolecular affinity interaction with lectin or antibody. And the third type is based on the chemical covalent coupling to free side groups of surface‐exposed proteins or carbohydrate chains, such as primary amines, carboxyl groups, glycan side chains. In addition, metabolic labeling and enzymatic reaction‐based methods have also been employed to selectively isolate cell surface proteins. In this review, we will provide a comprehensive overview of the enrichment methods for cell surface proteome profiling.