One-Stage Method for Assay of Tissue Factor Activity of Leukemic Cell with Special Reference to Disseminated Intravascular Coagulation. Issue 5 (27th January 2016)

• Record Type: Journal Article
• Authors: Andoh, Kiyoshi; Sadakata, Hiroto; Uchiyama, Toshimasa; Narahara, Nobuhiro; Tanaka, Hiroshi; Kobayashi, Norio; Maekawa, Tadashi
• Is Part Of: American journal of clinical pathology. Volume 93:Issue 5(1990)
• Page Start: 679
• Page End: 684
• Publication Date: 2016-01-27
• Subjects: Acute nonlymphoid leukemia -- Disseminated intravascular coagulation -- Tissue factor activity -- One-stage clotting assay -- Two-stage clotting assay -- Heparin therapy; Diagnosis, Laboratory -- Periodicals; Pathology -- Periodicals; 616.07
• DOI: 10.1093/ajcp/93.5.679
• Languages: English
• ISSNs: 0002-9173

Abstract

Abstract : Tissue factor activity (TFA) of leukemic cells (1 × 10 8 cells/mL) was measured in 44 patients with acute nonlymphoid leukemia (ANLL) by the one-stage assay using factor-IX deficient plasma (OSA-dIX) and two-stage assay (TSA). According to the preventative heparin dose schedule based on the TFA measured by the TSA, all disseminated intravascular coagulation (DIC) was controlled successfully. The procedure of the TSA was too complicated for clinical use, and its minimal measurable value was 125 units (U)/L of TFA. The OSA-dIX was simpler in its procedure and sensitive enough to measure accurately a TFA quantity as small as 30 U/L with high reproducibility. In 20 ANLL patients with 125 U/L or more of TFA measured by both assays, there was a significant relationship between their logarithms of TFA (r = 0.93, P < 0.01). These results suggested that DIC complication in ANLL patients would be controlled successfully by the administration of heparin dosage based on the TFA measured by the OSA-dIX.