Fecal stress, nutrition and reproductive hormones for monitoring environmental impacts on tigers (Panthera tigris). Issue 1 (12th January 2020)

• Record Type: Journal Article
• Authors: Mondol, Samrat; Booth, Rebecca K; Wasser, Samuel K
• Editors: Cooke, Steven
• Is Part Of: Conservation physiology. Volume 8:Issue 1(2020)
• Publication Date: 2020-01-12
• Subjects: Adrenal -- conservation physiology -- stress-nutrition interplay -- thyroid and reproductive hormones assay validation -- tigers; Nature -- Effect of human beings on -- Periodicals; Conservation biology -- Periodicals; 577.05
• DOI: 10.1093/conphys/coz091
• Languages: English
• ISSNs: 2051-1434

Abstract

Abstract : Glucocorticoid, thyroid, progesterone and androgen hormones are shown to reliably reflect biological activity of adrenal, thyroid and gonadal activation in captive tigers. Combined ACTH and TSH challenges further suggest how adrenal and thyroid hormone secretion complement one another to coordinate the physiological response to nutritional stress. Abstract: Non-invasive stress and nutritional hormone analysis in relation to ecological and other biological indices have tremendous potential to address environmental disturbance impacts on wildlife health. To this end, we examined the relation between glucocorticoid (GC) and thyroid (T3) hormone indices of disturbance and nutritional stress in response to ACTH and TSH challenges in captive tigers, as well as how reproductive hormones vary by sex and reproductive condition. Glucocorticoid, thyroid, progesterone and androgen assays conducted on high-performance liquid chromatography separated fractions of biologically relevant fecal extracts revealed high cross-reactivity of these assays for their respective biologically relevant fecal hormone metabolites. Both adrenal and thyroid hormone metabolites were elevated in response to ACTH and TSH challenges. However, the adrenal and thyroid hormone responses to ACTH challenge were concurrent, whereas the adrenal response to TSH challenge was delayed relative to thyroid hormone elevation in both males and females. The concurrently elevated T3 in response to ACTH may serve to raise metabolic rate to maximize use of GC-mobilized glucose, whereas the relatively delayed GC rise following TSH challenge may be a response to glucose depletion due to increased metabolic rate associated with elevated T3. Progesterone, testosterone and androstenedione hormone metabolites were significantly elevated during gestation compared to lactation in a female monitored from conception through early lactation. Results suggest that the glucocorticoid, thyroid and reproductive hormone assays we tested can accurately measure the stress, nutrition and reproductive response from tiger feces, providing useful non-invasive tools to assess physiological responses to environmental stressors and their reproductive consequences in the wild.