Efficient electrochemiluminescence of perylene nanocrystal entrapped in hierarchical porous Au nanoparticle-graphene oxide film for bioanalysis based on one-pot DNA amplification. (1st February 2020)

• Record Type: Journal Article
• Authors: Zhao, Jing; Da, Jun; Yang, Shan-Shan; Lei, Yan-Mei; Chai, Ya-Qin; Yuan, Ruo; Zhuo, Ying
• Is Part Of: Electrochimica acta. Volume 332(2020)
• Publication Date: 2020-02-01
• Subjects: Perylene nanocrystal -- Electrochemiluminescence -- Hierarchical porous film -- Target-catalyzed hairpin assembly -- MicroRNA-21; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2019.135389
• Languages: English
• ISSNs: 0013-4686

Abstract

Abstract: Although the perylene (Pe) affords the high quantum efficiency, its electrochemiluminescence (ECL) depends on organic solvents because of the serious aggregation-caused quenching effect in aqueous media, which impedes its application in ECL biological analysis. In this work, the hierarchical porous Au nanoparticle-graphene oxide nanocomposites(Au-GO) were prepared by using oleylamine as a reductant to generate Au nanoparticles which are intercalant to extend the intersheets distance of graphene oxide. Then the Au-GO could act as matrix for perylene nanocrystals (PeNCs) to load via π-π stacking which could prevent PeNCs from the self-aggregation to achieve an intense ECL of PeNCs in aqueous media. Consequently, a novel ternary ECL system was established with the perylene nanocrystals entrapped in Au-GO as ECL emitter, H2 O2 as coreactant and hemin/G-quadruplex as a coreaction accelerator. Furthermore, combined with the one-pot target-catalyzed hairpin assembly induced strand displacement amplification strategy, we proposed a sensitive sensing method to achieve microRNA (miRNA) promising detection with a limit of detection down to 3.3 aM and a wide detection range from 0.01 fM to 10 pM. Importantly, this work provides a new direction for solving the self-aggregation of organic ECL luminophores. Highlights: A new approach was applied for further preventing self-aggregation of PeNCs in the aqueous media. Application of typical planar PAHs with ACQ was enlarged in ECL biosensor. One-pot amplification strategy for target circular exponential amplification.