A photoactivatable microRNA probe for identification of microRNA targets and light-controlled suppression of microRNA target expression. Issue 4 (13th December 2019)

• Record Type: Journal Article
• Authors: Chen, Lei; Sun, Yu; Li, Jinbo; Zhang, Yan
• Is Part Of: Chemical communications. Volume 56:Issue 4(2020)
• Page Start: 627
• Page End: 630
• Publication Date: 2019-12-13
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c9cc08277h
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A dual-functional microRNA probe was constructed for identification of microRNA targets and regulation of microRNA function through light control. Abstract : Here, we report a novel dual-functional microRNA (miRNA) probe, PA-miRNA, for miRNA target identification and light control of miRNA target expression. PA-miRNA is a miRNA mimic with a 3′-biotin tag linked via a photo-cleavable linker. Using PA-miR-34a, intracellular targets of miR-34a in HeLa cells were isolated and confirmed. Moreover, PA-miR-34a upon transfection into HeLa cells was inactive until light irradiation to break the photo-cleavable linker to release functional miR-34a. We demonstrated that miR-34a target expression as well as miR-34a-promoted cell apoptosis were regulated by PA-miR-34a in a photo-controllable manner.