Daf-16/FOXO isoform b in AIY neurons is involved in low preference for Bifidobacterium infantis in Caenorhabditis elegans. (January 2020)

• Record Type: Journal Article
• Authors: Sun, Simo; Ohta, Akane; Kuhara, Atsushi; Nishikawa, Yoshikazu; Kage-Nakadai, Eriko
• Is Part Of: Neuroscience research. Volume 150(2020)
• Page Start: 8
• Page End: 16
• Publication Date: 2020-01
• Subjects: MAPK mitogen-activated protein kinase -- FOXO forkhead box-containing protein, O subfamily -- IGFR insulin-like growth factor receptor -- PTEN phosphatase and tensin homolog; Caenorhabditis elegans -- daf-16 -- Food preference -- Bifidobacterium infantis; Neurosciences -- Research -- Periodicals; Neurosciences -- Research -- Japan -- Periodicals; Neurology -- Periodicals; Neurosciences -- Periodicals; Neurosciences -- Recherche -- Périodiques; Neurosciences -- Recherche -- Japon -- Périodiques; Neurosciences -- Research; Japan; Periodicals; 612.8
• DOI: 10.1016/j.neures.2019.01.011
• Languages: English
• ISSNs: 0168-0102

Abstract

Highlights: C. elegans shows a low preference for Bifidobacterium infantis . Leaving the B. infantis lawn is likely to involve the AIY interneurons. daf-16 /FOXO isoform b is involved in the lawn-leaving behavior. Abstract: The neural and molecular mechanisms underlying food preference have been poorly understood. We previously showed that Bifidobacterium infantis ( B. infantis ), a well-known probiotic bacterium, extends the lifespan of Caenorhabditis elegans ( C. elegans ) compared with a standard food, Escherichia coli ( E. coli ) OP50. In this study, we characterized C. elegans behavior against B. infantis and examined the neural and molecular mechanisms governing that behavior. The majority of the wild-type animals were outside of the B. infantis lawn 10 min after transfer. Although worms did not prefer B. infantis compared to E. coli OP50, they preferred the B. infantis lawn over a lawn containing M9 buffer alone, in which there was no food. Mutant analyses suggested that leaving the B. infantis lawn required daf-16 /FOXO. Isoform-specific mutant phenotypes suggested that daf-16 isoform b seemed to be associated with leaving. Genetic rescue experiments demonstrated that the function of daf-16b in AIY interneurons was involved in leaving the B. infantis lawn. The daf-18 /PTEN mutants were also defective in leaving. In conclusion, C. elegans showed a low preference for B. infantis, and daf-16b in AIY interneurons and daf-18 had roles in leaving B. infantis .