Mitochondriotropic action and DNA protection: Interactions between phenolic acids and enzymes. Issue 1 (12th November 2019)

• Record Type: Journal Article
• Authors: de Abreu, Tatiane Silva; Braga, Mariana Aparecida; Simão, Anderson Assaid; Trento, Marcus Vinicius Cardoso; Eleutério, Mateus Willian de Faria; Silva Pereira, Luciana Lopes; da Cunha, Elaine Fontes Ferreira; Marcussi, Silvana
• Is Part Of: Journal of biochemical and molecular toxicology. Volume 34:Issue 1(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-11-12
• Subjects: comet assay -- enzymatic inhibitor -- genotoxicity -- phenolic compounds -- snake venom -- succinate dehydrogenase; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Toxicology -- Periodicals; 574
• DOI: 10.1002/jbt.22417
• Languages: English
• ISSNs: 1095-6670

Abstract

Abstract: The protective action of caffeic (CA) and syringic (SA) acids on the genotoxicity exercised by snake venoms was investigated in this study. Molecular interactions between phenolic acids and the enzyme succinate dehydrogenase were also explored. In the electrophoresis assay, SA did not inhibit the genotoxicity induced by the venom. However, CA partially inhibited DNA degradation. In the comet assay, SA and CA exerted an inhibitory effect on the venom‐induced fragmentation. Succinate dehydrogenase presented, in computational analyzes, favorable energies to the molecular bond to both the malonic acid and the phenolic compounds evaluated. In the enzymatic activity assays, SA inhibited succinate dehydrogenase and interfered in the interaction of malonic acid. Meanwhile, CA potentiated the inhibition exerted by the malonic acid. The results suggest transient interactions between toxins present in venoms and phenolic acids, mainly by hydrogen interactions, which corroborate with the data from previous works.