An efficient ruthenium(ii) tris(bipyridyl)-based chemosensor for the specific detection of cysteine and its luminescence imaging in living zebrafish. (12th November 2019)

• Record Type: Journal Article
• Authors: Li, Yibin; Shi, Ningning; Li, Minna
• Is Part Of: New journal of chemistry. Volume 43:Number 47(2019)
• Page Start: 18517
• Page End: 18524
• Publication Date: 2019-11-12
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c9nj04426d
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : A water-soluble, red emissive and cysteine-specific probe has been achieved through 1, 4-addition of cysteine to α, β-unsaturated ketones. Abstract : A novel luminescent chemosensor based on an α, β-unsaturated ketone conjugated Ru(bpy)3 2+ derivative (Ru-E ) has been designed and synthesized for the highly selective and sensitive detection of cysteine in 100% aqueous solutions. Owing to the conjugate 1, 4-addition of cysteine to α, β-unsaturated ketones, the non-luminescent chemosensor Ru-E could specifically react with cysteine to form the corresponding product, Ru-Cys, accompanied by remarkable luminescence enhancement at 620 nm. Job's plot and mass spectrometry analysis indicate a 1 : 2 binding stoichiometry between Ru-E and Cys. The sensing mechanism is confirmed by NMR and mass and emission spectrometry. Moreover, Ru-E exhibited very low cytotoxicity and was successfully applied to image endogenous thiols both in living bone mesenchymal stem cells (BMSCs) and zebrafish. In addition, Ru-E showed biological application for monitoring thiols in newborn-calf serum. The results of this work demonstrate that Ru-E would be a useful tool for physiological and pathological studies involving biothiols.