Human Papillomavirus (HPV) 16 and 18/45 Genotyping-Directed Follow-up of Women With Messenger RNA HPV-Positive, Cytology-Negative Cervical Screening Test Results. Issue 2 (11th October 2019)

• Record Type: Journal Article
• Authors: Han, Min; Li, Juan; Austin, Marshall; Varma, Kavita R; Zhang, Huina; Zhao, Chengquan
• Is Part Of: American journal of clinical pathology. Volume 153:Issue 2(2020)
• Page Start: 243
• Page End: 250
• Publication Date: 2019-10-11
• Subjects: Pap-negative cytology -- HPV test -- HPV genotyping -- Aptima; Diagnosis, Laboratory -- Periodicals; Pathology -- Periodicals; 616.07
• DOI: 10.1093/ajcp/aqz156
• Languages: English
• ISSNs: 0002-9173

Abstract

Abstract: Objectives: In this study, we sought to correlate genotype test results for human papillomavirus (HPV) types 16, 18, and 45 with histopathologic follow-up diagnoses in patients with messenger RNA (mRNA) high-risk HPV-positive, cytology-negative results. Methods: We identified 1, 157 patients with mRNA HPV-positive, cytology-negative cervical screening test results between June 2015 and June 2018. Reflex HPV 16/18/45 genotype results were documented in 1, 018 women aged 30 years or older, 318 of whom had follow-up within 18 months. Results: Histopathologic findings of cervical intraepithelial neoplasia 2 or worse (CIN2+) were diagnosed in 14 of 122 (11.5%) patients positive for HPV 16/18/45 vs in seven of 196 (3.6%) HPV 16/18/45–negative patients. Three patients with high-risk HPV–positive, cytology-negative cervical screening test results were diagnosed with stage I cervical adenocarcinomas following early colposcopic referral and biopsy after HPV 16/18/45–positive genotype results. Conclusions: Immediate reflex HPV 16/18/45 genotyping of mRNA HPV-positive, cytology-negative patients led to early colposcopic referral and histopathologic diagnoses of three difficult-to-detect, low-stage, cervical adenocarcinomas and significantly increased overall early detection of CIN2+ lesions.