Photoinduced inhibition of DNA repair enzymes and the possible mechanism of photochemical transformations of the ruthenium nitrosyl complex [RuNO(β-Pic)2(NO2)2OH]. Issue 12 (26th September 2019)

• Record Type: Journal Article
• Authors: Mikhailov, Artem A.; Khantakova, Darya V.; Nichiporenko, Vladislav A.; Glebov, Evgeni M.; Grivin, Vjacheslav P.; Plyusnin, Victor F.; Yanshole, Vadim V.; Petrova, Daria V.; Kostin, Gennadiy A.; Grin, Inga R.
• Is Part Of: Metallomics. Volume 11:Issue 12(2019)
• Page Start: 1999
• Page End: 2009
• Publication Date: 2019-09-26
• Subjects: Metals -- Physiological effect -- Periodicals; 572.51
• DOI: 10.1039/c9mt00153k
• Languages: English
• ISSNs: 1756-5901

Abstract

Abstract : Inhibition of DNA repair enzymes by the ruthenium nitrosyl complex occurs only after light irradiation and is determined by the interactions between the enzyme and active photolysis products. Abstract : In this work we have demonstrated that the ruthenium nitrosyl complex [RuNO(β-Pic)2 (NO2 )2 OH] is suitable for investigation of the inactivation of DNA repair enzymes in vitro . Photoinduced inhibition of DNA glycosylases such as E. coli Endo III, plant NtROS1, mammalian mNEIL1 and hNEIL2 occurs to an extent of ≥90% after irradiation with the ruthenium complex. The photophysical and photochemical processes of [RuNO(β-Pic)2 (NO2 )2 OH] were investigated using stationary and time-resolved spectroscopy, and mass spectrometry. A possible mechanism of the photo-processes was proposed from the combined spectroscopic study and DTF calculations, which reveal that the photolysis is multistage. The primary and secondary photolysis stages are the photo-induced cleavage of the Ru–NO bond with the formation of a free nitric oxide and Ru III complex followed by ligand exchange with solvent. For E. coli Endo III, covalent interaction with the photolysis product was confirmed by UV-vis and mass spectrometric methods.