Survey of pathogenic bacteria of biofilms in a metropolitan drinking water distribution system. Issue 20 (7th November 2019) - Chen, Jiang
ABSTRACT: Bacteria, especially pathogenic bacteria, were detected in order to estimate the safety of drinking water distribution systems (DWDSs). Sixteen biofilms and 12 water samples (six retained and six flowing) were collected from a city DWDS in eastern China. Biofilms were observed using scanning electron microscopy. Cultivable bacteria of biofilms were counted by heterotrophic plate counts, ranging from 3.61 × 10 1 to 1.67 × 10 6 CFU·cm −2 . Coliforms, Salmonella, Shigella, Vibrio and Legionella were separated by Eosin-Methylene Blue (EMB) agar, Salmonella chromogenic medium, Shigella chromogenic medium, Thiosulfate Citrate Bile Salts Sucrose (TCBS) agar and Buffered Charcoal Yeast Extract (BCYE) agar and 13/16, 8/16, 7/16, 6/16, 0/16 biofilm samples were found to be positive, respectively. Retained and flowing water samples were collected to estimate the influence of hydrodynamic conditions on biofilm detachment. All six retained water samples were positive for bacteria, the count ranged from 1.2 × 10 3 to 2.8 × 10 4 CFU·mL −1 and 2/6, 3/6, 2/6, 0/6, 0/6 samples were positive for coliforms, Salmonella, Shigella, Legionella and Vibrio, respectively. While only three of six flowing water samples were bacteria positive, the counts ranged from 10 2 to 10 3 CFU·mL −1, 2/6 were coliform positive and no pathogens were detected under testing. The results show that there are pathogens in DWDS biofilms, which can cause health-related problems if detached from their surfaces. Abstract : In this study, 16 biofilms and 12 water samples from metropolitan drinking water distribution systems were collected and screened for pathogenic bacteria in order to estimate the hygiene and safety of drinking water distribution systems.